Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats
장혜윤, Oh Seo-Eun, 오승한, 허경석, 김성태,
소속 상세정보
장혜윤 ( Chang Hye-Yoon ) - Seoul National University School of Dentistry Department of Periodontology
( Oh Seo-Eun ) - Seoul National University School of Dentistry Department of Periodontology
오승한 ( Oh Seung-Han ) - Wonkwang University College of Dentistry Department of Oral and Maxillofacial Surgery
허경석 ( Hu Kyung-Seok ) - Yonsei University College of Dentistry Department of Oral Biology
김성태 ( Kim Sung-Tae ) - Seoul National University School of Dentistry Department of Periodontology
KMID : 0363020160460040244
Abstract
Purpose: The aim of this study was to characterize the healing in the grafted calvarial defects of rats after adjunctive hyperbaric oxygen therapy.
Methods: Twenty-eight male Sprague-Dawley rats (body weight, 250?300 g) were randomly divided into two treatment groups: with hyperbaric oxygen therapy (HBO; n=14) and without HBO (NHBO; n=14). Each group was further subdivided according to the bone substitute applied: biphasic calcium phosphate (BCP; n=7) and surface-modified BCP (mBCP; n=7). The mBCP comprised BCP coated with Escherichia-coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Two symmetrical circular defects (6-mm diameter) were created in the right and left parietal bones of each animal. One defect was assigned as a control defect and received no bone substitute, while the other defect was filled with either BCP or mBCP. The animals were allowed to heal for 4 weeks, during which those in the HBO group underwent 5 sessions of HBO. At 4 weeks, the animals were sacrificed, and the defects were harvested for histologic and histomorphometric analysis.
Results: Well-maintained space was found in the grafted groups. Woven bone connected to and away from the defect margin was formed. More angiogenesis was found with HBO and EGCG/BMP-2 (P<0.05). None of the defects achieved complete defect closure. Increased new bone formation with HBO or EGCG/BMP-2 was evident in histologic evaluation, but it did not reach statistical significance in histometric analysis. A synergic effect between HBO and EGCG/BMP-2 was not found.
Conclusions: Within the limitations of this study, the present findings indicate that adjunctive HBO and EGCG/BMP-2 could be beneficial for new bone formation in rat calvarial defects.
키워드
Biphasic calcium phosphate; BMP-2; Bone substitute; EGCG; Hyperbaric oxygen ther
원문 및 링크아웃 정보
등재저널 정보